Journal: Communications biology

Article Title: The distinct transcriptomic signature of the resolution phase fibroblast-like synoviocytes supports endothelial cell dysfunction.

doi: 10.1038/s42003-025-08250-1

Figure Lengend Snippet: Fig. 1 | Inflammation in distal interphalangeal joints of iTNF-tg was reversible at the histopathological level. A Schematic drawing describing the experimental design of doxycycline-inducible human TNFα–transgenic (iTNF-tg) arthritis mice model. Inflammation phase mice were treated with 1 mg/ml doxycycline and 5% sucrose for 3 weeks; Resolution phase mice were treated with 1 mg/ml doxycycline and 5% sucrose for an initial 3 weeks, after which the antibiotic was removed for the next 3 weeks; The homeostasis group included untreated control mice which were kept on only sugar (5% sucrose) water for 3 weeks. B Images of toluidine blue staining. Synovitis (asterisks) and cartilage destaining (arrow) were observed in the inflammation group. Scale bar = 100 μm. C Quantification of inflammation by estimation of No. of synoviocytes/106 sq.µm (left) and cartilage destaining (right) by histomorphometry (n = 5). ∗∗p < 0.01; ∗∗∗∗p < 0.0001. Values in C are shown in mean ± SD. D Representative images of F8/40 and (E) CD31 immuno-stained sections. Scale bar: 100 μm. Samples were counterstained with Fast Green FCF.

Article Snippet: All slides then were incubated at 4 °C overnightwith primary antibodies anti-CD31 (1:200, 77699,Cell Signaling), and anti F4/80 (1:200, 70076, Cell Signaling).

Techniques: Transgenic Assay, Control, Staining

Journal: Communications Biology

Article Title: The distinct transcriptomic signature of the resolution phase fibroblast-like synoviocytes supports endothelial cell dysfunction

doi: 10.1038/s42003-025-08250-1

Figure Lengend Snippet: A Schematic drawing describing the experimental design of doxycycline-inducible human TNFα–transgenic (iTNF-tg) arthritis mice model. Inflammation phase mice were treated with 1 mg/ml doxycycline and 5% sucrose for 3 weeks; Resolution phase mice were treated with 1 mg/ml doxycycline and 5% sucrose for an initial 3 weeks, after which the antibiotic was removed for the next 3 weeks; The homeostasis group included untreated control mice which were kept on only sugar (5% sucrose) water for 3 weeks. B Images of toluidine blue staining. Synovitis (asterisks) and cartilage destaining (arrow) were observed in the inflammation group. Scale bar = 100 μm. C Quantification of inflammation by estimation of No. of synoviocytes/10 6 sq.µm (left) and cartilage destaining (right) by histomorphometry ( n = 5). ∗∗ p < 0.01; ∗∗∗∗ p < 0.0001. Values in C are shown in mean ± SD. D Representative images of F8/40 and ( E ) CD31 immuno-stained sections. Scale bar: 100 μm. Samples were counterstained with Fast Green FCF.

Article Snippet: To reduce further nonspecific background staining, slides were incubated with 3% normal goat serum for 20 min. All slides then were incubated at 4 °C overnight with primary antibodies anti-CD31 (1:200, 77699, Cell Signaling), and anti F4/80 (1:200, 70076, Cell Signaling).

Techniques: Transgenic Assay, Control, Staining